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perk1 2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc perk1 2

    Perk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 16851 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+p44+42+mapk/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+Antibody/pmc13669479-12-0-8
    Average 99 stars, based on 16851 article reviews
    perk1 2 - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Fc‐Dependent Targeting of the GAS6–MERTK Axis With Monoclonal and Bispecific Antibodies Overcomes Drug Resistance Across EGFR Mutations in Exons 19, 20, and 21"

    Article Title: Fc‐Dependent Targeting of the GAS6–MERTK Axis With Monoclonal and Bispecific Antibodies Overcomes Drug Resistance Across EGFR Mutations in Exons 19, 20, and 21

    Journal: Advanced Science

    doi: 10.1002/advs.78290


    Figure Legend Snippet:

    Techniques Used: Cell-Signaling

    Related Articles

    Staining:

    Article Title: Colchicine acutely reduces liver-secreted inflammatory and metabolic proteins in blood.
    Article Snippet: Proteins were then separated on NuPage 4-12% Bis-Tris gels (Invitrogen) by SDS-page electrophoresis. .. The proteins were transferred onto PVDF membranes and stained with phospho-p44/42 MAPK (#9101, CST) and p44/42 MAPK (#9102, CST), phopsho-SAPK/JNK (#4668, CST), SAPK/JNK (#9252, CST), GAPDH (#5174, CST) primary antibodies at 1:1000 dilution. .. Next day, the membranes were washed 3 times with TBS-T (20 mM Tris-HCl pH 7.5, 150 mM NaCl and 0.1% (v/v) Tween-20) and incubated HRP-linked anti-rabbit IgG (#7074, CST) secondary Jo rn l P repro of 42 antibody for 1 hour at room temperature.

    Article Title: Engineered pistol ribozymes selectively target KRAS G12V with enhanced efficacy by capping modification.
    Article Snippet: Technology Engineered pistol ribozymes selectively target KRAS G12V with enhanced efficacy by capping modification

    other:

    Article Title: Placing purines in precision medicine: Targeting a metabolic reliance in KRAS -mutant tumors
    Article Snippet: Phospho-p44/42 MAPK (Erk1/2) , CST , Cat#9106S; RRID: AB_331768 ).

    Cell-Signaling:

    Article Title: Carica papaya leaf and root extracts attenuate hyperglycemia-induced insulin resistance by modulating MAPK and PI3K/AKT signalling in hepatic and skeletal muscle cells
    Article Snippet: p44/42 MAPK (ERK1/2) , Total , Rabbit , 4348S , Cell Signaling Technology (USA) , 1:1000. .. Phospho-p44/42 MAPK (Thr202/Tyr204) , Phospho-specific , Rabbit , 8544S , Cell Signaling Technology (USA) , 1:1000. .. p38 MAPK , Total , Rabbit , 8690S , Cell Signaling Technology (USA) , 1:1000.

    Article Title: Docetaxel treatment upregulates estrogen receptor expression in triple negative breast cancer cells via activating arachidonic acid pathway
    Article Snippet: .. The membranes were blocked with 3% bovine serum albumin (BSA) to prevent nonspecific binding and incubated with primary antibodies: anti-Estrogen Receptor alpha antibody (ab75635, Abcam), Cleaved caspase 3 (9661, Cell Signaling Technology, CST), c-Jun (9165, CST), Phospho-c-Jun (3270, CST), Phospho-SAPK/JNK (4668, CST), Phospho-p38 MAPK (4511, CST), Phospho-p44/42 MAPK (Erk1/2, 4370, CST), and β-Actin antibody (4967, CST). .. After incubated with appropriate secondary antibodies, the bands were visualized using Clarity Western ECL Substrate (Bio-Rad) and imaged using a chemiluminescence detection system with auto-exposure setting.

    Immunohistochemistry:

    Article Title: Engineered pistol ribozymes selectively target KRAS G12V with enhanced efficacy by capping modification.
    Article Snippet: Technology Engineered pistol ribozymes selectively target KRAS G12V with enhanced efficacy by capping modification

    Bioprocessing:

    Article Title: CXCR4, CXCR7 and PBRM1 are responsible for everolimus and cabozantinib resistance in human renal cancer cells.
    Article Snippet: .. Rabbit monoclonal antibodies for p44/42 MAPK (ERK1/2) (Cat# 9102, RRID:AB_330744), phospho-p44/42 MAPK (ERK1/2; T202/Y204) (Cat# 9101, RRID:AB_331646), phospho-4EBP1 (T70) (Cat# 9455, RRID:AB_330949), 4EBP1 (53H11) (Cat# 9644, RRID:AB_2097841), pMET (Y1349) (Cat# 3133, RRID:AB_2181539), mouse monoclonal antibody for MET (L41G3) (Cat# 3148, RRID:AB_1031042), YY1 (D5D9Z) rabbit monoclonal antibody (Cat# 46395, RRID:AB_2799302) were obtained from Cell Signaling (Danvers, Massachusetts, USA). .. Secondary antibody includes goat anti-rabbit-HRP (Jackson ImmunoResearch, West Grove, Pennsylvania, USA) and goat anti-mouse-HRP (Proteintech, Rosemont, Illinois, USA).

    Membrane:

    Article Title: Sphingosine-1-phosphate drives astrocyte pyroptosis via activation of NLRC4 inflammasome in autism spectrum disorder.
    Article Snippet: .. The membrane was washed three times with TBS-Tween 20, each wash lasting 5 min, and then incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (Thermo Fisher Scientific, 31460) or goat anti-mouse IgG (Thermo Fisher Scientific, 31430) at room temperature for 1 h. Antibody details are provided below, NLRC4 (ab201792,1:1000,Abcam), Pro-Caspase-1 (AF5418, Affinity), Caspase-1 (AF4005, 1:1000, Affinity), Pro-GSDMD (AF4012, 1:1000, Affinity), GSDMD (DF13758, 1:1000, Affinity), GFAP (3670S, 1:1000, Cell Signaling), phospho-p44/42 MAPK (4370S, 1:2000, Cell Signaling), p44/42 MAPK(Erk1/2) (4695 s, 1:2000, Cell Signaling), JNK (66210–1-Ig, 1:1000, Proteintech), Phospho-JNK (Tyr185) (80024–1- RR, 1:1000, Proteintech), p38 MAPK (14064–1-AP, 1:1000, Proteintech), Phospho-p38 MAPK (28796–1-AP, 1:1000, Proteintech), GAPDH (TA802519, 1:2000, Origene). .. Specific protein bands were detected using an enhanced chemiluminescence (ECL) kit (Meilunbio, MA0186).

    Incubation:

    Article Title: Sphingosine-1-phosphate drives astrocyte pyroptosis via activation of NLRC4 inflammasome in autism spectrum disorder.
    Article Snippet: .. The membrane was washed three times with TBS-Tween 20, each wash lasting 5 min, and then incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (Thermo Fisher Scientific, 31460) or goat anti-mouse IgG (Thermo Fisher Scientific, 31430) at room temperature for 1 h. Antibody details are provided below, NLRC4 (ab201792,1:1000,Abcam), Pro-Caspase-1 (AF5418, Affinity), Caspase-1 (AF4005, 1:1000, Affinity), Pro-GSDMD (AF4012, 1:1000, Affinity), GSDMD (DF13758, 1:1000, Affinity), GFAP (3670S, 1:1000, Cell Signaling), phospho-p44/42 MAPK (4370S, 1:2000, Cell Signaling), p44/42 MAPK(Erk1/2) (4695 s, 1:2000, Cell Signaling), JNK (66210–1-Ig, 1:1000, Proteintech), Phospho-JNK (Tyr185) (80024–1- RR, 1:1000, Proteintech), p38 MAPK (14064–1-AP, 1:1000, Proteintech), Phospho-p38 MAPK (28796–1-AP, 1:1000, Proteintech), GAPDH (TA802519, 1:2000, Origene). .. Specific protein bands were detected using an enhanced chemiluminescence (ECL) kit (Meilunbio, MA0186).

    Article Title: Docetaxel treatment upregulates estrogen receptor expression in triple negative breast cancer cells via activating arachidonic acid pathway
    Article Snippet: .. The membranes were blocked with 3% bovine serum albumin (BSA) to prevent nonspecific binding and incubated with primary antibodies: anti-Estrogen Receptor alpha antibody (ab75635, Abcam), Cleaved caspase 3 (9661, Cell Signaling Technology, CST), c-Jun (9165, CST), Phospho-c-Jun (3270, CST), Phospho-SAPK/JNK (4668, CST), Phospho-p38 MAPK (4511, CST), Phospho-p44/42 MAPK (Erk1/2, 4370, CST), and β-Actin antibody (4967, CST). .. After incubated with appropriate secondary antibodies, the bands were visualized using Clarity Western ECL Substrate (Bio-Rad) and imaged using a chemiluminescence detection system with auto-exposure setting.

    Nonspecific Binding:

    Article Title: Docetaxel treatment upregulates estrogen receptor expression in triple negative breast cancer cells via activating arachidonic acid pathway
    Article Snippet: .. The membranes were blocked with 3% bovine serum albumin (BSA) to prevent nonspecific binding and incubated with primary antibodies: anti-Estrogen Receptor alpha antibody (ab75635, Abcam), Cleaved caspase 3 (9661, Cell Signaling Technology, CST), c-Jun (9165, CST), Phospho-c-Jun (3270, CST), Phospho-SAPK/JNK (4668, CST), Phospho-p38 MAPK (4511, CST), Phospho-p44/42 MAPK (Erk1/2, 4370, CST), and β-Actin antibody (4967, CST). .. After incubated with appropriate secondary antibodies, the bands were visualized using Clarity Western ECL Substrate (Bio-Rad) and imaged using a chemiluminescence detection system with auto-exposure setting.



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    Image Search Results


    Journal: Advanced Science

    Article Title: Fc‐Dependent Targeting of the GAS6–MERTK Axis With Monoclonal and Bispecific Antibodies Overcomes Drug Resistance Across EGFR Mutations in Exons 19, 20, and 21

    doi: 10.1002/advs.78290

    Figure Lengend Snippet:

    Article Snippet: pERK1/2 (Thr202/Tyr204) , 9101S , Cell Signaling Technology (CST) , AB_331646.

    Techniques: Cell-Signaling